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Biokonversi Senyawa Karsinogenik Benzo (a) Antrasen Oleh Bakteri Laut Pseudomonas sp. KalP3b22 yang Diisolasi dari Air Laut Pelabuhan Kumai, Kalimantan Tengah
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Murniasih, Tutik; Yopi; Budiawan, 2018, "Biokonversi Senyawa Karsinogenik Benzo (a) Antrasen Oleh Bakteri Laut Pseudomonas sp. KalP3b22 yang Diisolasi dari Air Laut Pelabuhan Kumai, Kalimantan Tengah", https://hdl.handle.net/20.500.12690/RIN/A9NRVT, RIN Dataverse, V1
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Description

Salah satu usaha untuk mendetoksifikasi senyawa karsinogenik benzo(a)antrasen di lingkungan laut yaitu dengan mempelajari biodegradasi dari senyawa tersebut. Mikroba laut berperan penting dalam anabolisme senyawa-senyawa poliaromatik sehingga dapat dimanfaatkan sebagai biodegradator. Tahap pertama sebelum suatu senyawa terdegradasi adalah terjadi konversi dari strukturnya untuk meningkatkan kepolarannya. Studi yang mempelajari pola degradasi benzo(a)antrasen oleh mikroba laut asal perairan Indonesia dilakukan untuk mengetahui kemampuannya dalam mendegradasi senyawa rekalsitran. Secara garis besar penelitian ini meliputi isolasi bakteri menggunakan media selektif , skrining bakteri pendegradasi PAH dengan metode sublimasi, uji degradasi dengan fermentasi skala laboratorium dan penentuan produk konversinya. Salah satu isolat potensial yang didapat dari penelitian ini adalah dari jenis Pseudomonas sp. asal Kumai dengan kode isolat KalP3b22. Isolat tersebut terbukti mampu mengkonversi benzo(a)antrasen menjadi struktur teroksidasi sesudah 56 hari kultivasi. Analisa struktur senyawa hasil konversi dengan metode spektroskopi menunjukkan bahwa benzo(a)antrasen terkonversi oleh bakteri ini menjadi benzo(a)antrasen 7,12 diol, yang selanjutnya teroksidasi lagi menjadi benzo(a)antrasen 7,12 dion.

OLDI, Vol. 34, No. 3.

(2008)
Subject Earth and Environmental Sciences
Keyword biokonversi, benzo(a)antrasen, Pseudomonas sp, Kumai
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