Application of organic biofertilizer is rising due to enhancement in nutrient uptake efficiency and society demands for more green technologies for sustainable agriculture development. The aims of this research were to production of organic biofertilizer powder starter and develop it’s technology that can adopted by industries. We improve a technology to produce biofertilizer powder starter, including bacterial fermentation in pilot-scale fermentor, cells harvesting with continuous centrifuge, freeze-drying, ,mixing carrier and packaging. We fermented three bacteria which is have the best potency for plant growth promoting, including Bacillus sp. 140B as biocontrol, Brevundimonas sp. AA2 with the highest growth hormone production, and Brevundimonas sp. Piko as phosphate solubilizer. To maintenance and enhance the viability and stability of the cells during freeze-drying process, we added combination of skim milk and trehalose as cryoprotectant agents. The cryoprotectants have a good protection for the viability of bacteria, were shown by stability of bacterial population. Carrier materials were used to make powder starter, including maltodextrin and maize starch.
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